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Anti-MBP, AlpSdAbs® VHH(HRP)

Details and Advantages
Applications: WB,ELISA
Reactivity: MBP
Conjugate: HRP
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description: 

Anti-MBP, AlpSdAbs® VHH(HRP) is designed for detecting MBP fusion proteins. Anti-MBP, AlpSdAbs® VHH(HRP) is based on monoclonal, recombinant, single domain antibody to MBP coupled to HRP, and Anti-MBP, AlpSdAbs® VHH(HRP) detects the MBP selectively, no reactivity with other proteins.

Immunogen: MBP fusion protein                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: MBP
Cross-Reactivity: Recognizes MBP specifically. Does not cross-react with other proteins.
Affinity: Dissociation constant KD of 0.1nM      
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.



性能 >

WB:       1:5,000-1:20000
ELISA:   1:5,000-1:20000


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.