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Anti-His tag, AlpSdAbs® VHH(Biotin)

Details and Advantages
Applications: ELISA,Purification,BLI,SPR
Reactivity: His tag
Conjugate: Biotin
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description: 

Anti-His tag, AlpSdAbs® VHH(Biotin) is designed for detecting His tag fusion proteins. Anti-His tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to His tag coupled to Biotin, and Anti-His tag, AlpSdAbs® VHH(Biotin) detects the His tag selectively, no reactivity with other proteins.


Immunogen: 6*His tag fusion protein                    
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: His tag sequence(HHHHHH)
Cross-Reactivity: Highly selective for His tag sequence
Affinity: Dissociation constant KD of 0.1nM      
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)




性能 >

WB:       1:5,000-1:20000
ELISA:    1:5,000-1:20000

IP:         1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.