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TG1 Super Electrocompetent Cells+Helper Phage

Details and Advantages
Applications: Phage Display
Advantages:

Transformation Efficiency ≥ 4 x 10^10 cfu/µg pUC19.

M13KO7 infected.

Control DNA included.

概述 >

Introduction:
TG1 Super Electrocompetent Cells+Helper Phage are TG1 Super Electrocompetent Cells which have already been infected with M13KO7. This strain has already been infected with M13KO7 and does not require further helper phage infection for use.
TG1 Super Electrocompetent Cells deliver ≥4 x 10^10 cfu/ug of DNA and are particularly useful for phage display protein expression. M13KO7 is an M13 derivative, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication. M13KO7 is able to replicate in the absence of phagemid DNA.

Specifications:
Competence: Electroporation Competent(M13KO7 infected)
Application: Phage Display
Organism: E. coli

Applications:
Phage display library construction and screening
Antibody epitope mapping
Peptide ligand identification
Protein-Protein interaction identification
Directed evolution of proteins
Find tumor antigens, candidates for therapeutic antibodies, enzyme inhibitors, receptor agonists, etc.

Features:
Transformation Efficiency ≥ 4 x 1010 cfu/µg pUC19.
M13KO7 infected.
Control DNA included.
Packaged as DUOs (2 transformations per tube)

Genotype:
[F' traD36 proA+B+ lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK- mK-)