Anti-V5 tag, AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                WB,ELISA,IP,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                V5 tag
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-V5 tag, AlpSdAbs® VHH(Biotin) is designed for detecting V5 tag fusion proteins. Anti-V5 tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to V5 tag coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-V5 tag, AlpSdAbs® VHH(Biotin) detects the V5 tag selectively, no reactivity with other proteins.

Immunogen: V5 tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: V5 tag sequence(GKPIPNPLLGLDST)
Cross-Reactivity: Highly selective for V5 tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.