Anti-TagFP, AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                ELISA,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                TagFP
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-TagFP, AlpSdAbs® VHH(Biotin) is designed for detecting TagFP fusion proteins. Anti-TagFP, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to TagFP coupled to Biotin, and Anti-TagFP, AlpSdAbs® VHH(Biotin) detects the TagFP selectively, no reactivity with other proteins.

Immunogen: TagFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: TagFP(TagRFP/TagBFP)
Cross-Reactivity: Highly selective for TagRFP/TagBFP. Does not cross-react with common GFP or dsRed derivatives.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

TagRFP is derived from the Entacmaea quadricolor fluorescent protein TurboRFP (a random mutant of eqFP578), with mutations of R162E, Q166D, S180N, F198V, F200Y at the hydrophilic interface. TagBFP was derived from TagRFP with the some mutations. TagRFP/TagBFP has a high fluorescent quantum yield (Φfluo 0.48) and is widely used for fluorescent imaging. For biochemical analysis including mass spectrometry and enzymeactivity measurements.

Performance >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000

IP: 1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.