Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                WB,ELISA,IP,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                Rabbit IgG(H+L)
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(Biotin) is designed for detecting rabbit IgG(H+L) specifically. Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibodies to rabbit IgG(H+L) coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(Biotin) reacts with rabbit IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant Rabbit IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin-SP (long spacer)
Specificity: Rabbit IgG(H+L)
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

ELISA: 1:10000-1:50000
WB: 1:10000-1:50000
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.