Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                WB,ELISA,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                Rabbit IgG(Fcγ Fragment specific)
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) is designed for detecting Fc region of rabbit IgG specifically. Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibodies to rabbit IgG (Fcγ Fragment specific) coupled to biotin, and the Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) reacts with the Fc region of rabbit IgG selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant Rabbit IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin-SP (long spacer)
Specificity: Fc region of Rabbit IgG
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG
Affinity: ＜1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Performance >

ELISA: 1:10000-1:50000
WB: 1:10000-1:50000

IP: 1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.