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Anti-Mouse IgG(H+L), AlpSdAbs® VHH(Biotin)

Details and Advantages
Applications: ELISA,Purification,BLI,SPR
Reactivity: Mouse IgG(H+L)
Conjugate: Biotin
Advantages:

·High lot-to-lot consistency

·Increased sensitivity and higher affinity

·Animal-free production

概述 >

Description: 

Anti-Mouse IgG(H+L), AlpSdAbs® VHH is designed for detecting mouse IgG(H+L) specifically. Anti-Mouse IgG(H+L), AlpSdAbs® VHH is monovalent, recombinant single domain antibodies derived from the variable regions of heavy chain of Alpaca pacous, and Anti-Mouse IgG(H+L), AlpSdAbs® VHH reacts with the heavy chain and light chain of mouse IgG selectively, no reactivity with rabbit, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant mouse IgG                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c    
Conjugate:  Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Mouse IgG(H+L)
Cross-Reactivity: No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG       
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

性能 >

ELISA:  1:10000-1:50000
WB:     1:10000-1:50000

IP:        1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.