Anti-Mouse IgG(H+L), AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                ELISA,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                Mouse IgG(H+L)
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                ·High lot-to-lot consistency
·Increased sensitivity and higher affinity
·Animal-free production

Summary >

Description:

Anti-Mouse IgG(H+L), AlpSdAbs® VHH is designed for detecting mouse IgG(H+L) specifically. Anti-Mouse IgG(H+L), AlpSdAbs® VHH is monovalent, recombinant single domain antibodies derived from the variable regions of heavy chain of Alpaca pacous, and Anti-Mouse IgG(H+L), AlpSdAbs® VHH reacts with the heavy chain and light chain of mouse IgG selectively, no reactivity with rabbit, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Mouse IgG(H+L)
Cross-Reactivity: No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Performance >

ELISA: 1:10000-1:50000
WB: 1:10000-1:50000

IP: 1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.