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Anti-Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH

Details and Advantages
Applications: WB,ELISA
Reactivity: Mouse IgG2a(Fcγ Fragment specific)
Conjugate: Unconjugated
Advantages:

·High lot-to-lot consistency

·Increased sensitivity and higher affinity

·Animal-free production

Summary >

Description: 

Anti-Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH is designed for detecting mouse IgG2a Fcγ fragment specifically. Anti-Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH is monovalent, recombinant single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH reacts with the Fc fragment of mouse IgG2a selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Immunogen: Recombinant Fc region of mouse IgG2a                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c    
Conjugate:  Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Mouse IgG2a (Fcγ fragment specific) 
Cross-Reactivity: No cross-reactivity with mouse IgG1/2b/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Affinity: <1nM      
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Performance >

ELISA:   1:10000-1:50000
WB:      1:10000-1:50000


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.