Anti-Human IgD, AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                ELISA,IP,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                Human IgD
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-Human IgD, AlpSdAbs® VHH(Biotin) is designed for detecting human IgD specifically. Anti-Human IgD, AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibody to human IgD coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgD, AlpSdAbs® VHH(Biotin) reacts with human IgD chain selectively, no reactivity with human IgG, IgA, IgM, IgE.

Immunogen: Human IgD
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin-SP (long spacer)
Specificity: Human IgD
Cross-Reactivity: Does not bind to human IgG, IgA, IgM, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

ELISA： 1:5000-1:20000
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.