Anti-His tag, AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                ELISA,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                His tag
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-His tag, AlpSdAbs® VHH(Biotin) is designed for detecting His tag fusion proteins. Anti-His tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to His tag coupled to Biotin, and Anti-His tag, AlpSdAbs® VHH(Biotin) detects the His tag selectively, no reactivity with other proteins.

Immunogen: 6*His tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: His tag sequence(HHHHHH)
Cross-Reactivity: Highly selective for His tag sequence
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Performance >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000

IP: 1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.