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Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)

Details and Advantages
Applications: WB,ELISA,Purification,BLI,SPR
Reactivity: Goat IgG(Fcγ Fragment specific)
Conjugate: Biotin
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

Summary >

Description: 

Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) is designed for detecting Fc region of goat IgG specifically. Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibodies to goat IgG(Fcγ Fragment specific) coupled to biotin, and the Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) reacts with the Fc region of goat IgG selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat IgG.


Immunogen: Recombinant Fc region of goat IgG                      
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c    
Conjugate: Biotin-SP (long spacer)
Specificity: Fc region of goat IgG
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG       
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Performance >

ELISA:  1:5000-1:20000
WB:     1:5000-1:20000

IP:        1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.