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Anti-GST, AlpSdAbs® VHH(Biotin)

Details and Advantages
Applications: ELISA,Purification,BLI,SPR
Reactivity: GST
Conjugate: Biotin
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description: 

Anti-GST, AlpSdAbs® VHH(Biotin) is designed for detecting GST fusion proteins. Anti-GST, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to GST coupled to Biotin, and Anti-GST, AlpSdAbs® VHH(Biotin) detects the GST selectively, no reactivity with other proteins.

Immunogen: GST fusion protein                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: GST
Cross-Reactivity: Highly selective for GST       
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)


Background:

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade. GST is one useful epitope ta for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.



性能 >

WB:       1:5,000-1:20000
ELISA:   1:5,000-1:20000

IP:         1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.