Anti-Human IgA, AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                ELISA,IP,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                Human IgA
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-Human IgA, AlpSdAbs® VHH(Biotin) is designed for detecting human IgA specifically. Anti-Human IgA, AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibody to human IgA coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgA, AlpSdAbs® VHH(Biotin) reacts with human IgA chain selectively, no reactivity with human IgG, IgE, IgM, IgD.

Immunogen: Human IgA
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin-SP (long spacer)
Specificity: Human IgA
Cross-Reactivity: Does not bind to human IgG, IgE, IgM, IgD
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgA is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

ELISA： 1:5000-1:20000
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.