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Anti-Human IgE, AlpSdAbs® VHH(Biotin)

Details and Advantages
Applications: ELISA,IP,Purification,BLI,SPR
Reactivity: Human IgE
Conjugate: Biotin
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description:
Anti-Human IgE, AlpSdAbs® VHH(Biotin) is designed for detecting human IgE specifically. Anti-Human IgE, AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibody to human IgE coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgE, AlpSdAbs® VHH(Biotin) reacts with human IgE chain selectively, no reactivity with human IgG, IgA, IgM, IgD.

Immunogen: Human IgE                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c    
Conjugate: Biotin-SP (long spacer)
Specificity: Human IgE
Cross-Reactivity: Does not bind to human IgG, IgA, IgM, IgD      
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

性能 >

ELISA: 1:5000-1:20000
IP:          1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.