Details and Advantages
Applications:
ICC/IF,Super-resolution microscopy
Reactivity:
Rabbit IgG
Conjugate:
AF647
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-Rabbit IgG kappa, AlpSdAbs® VHH(AF647 ×4) is designed for detecting rabbit IgG kappa chain in super-resolution microscopy techniques specifically. Anti-Rabbit IgG kappa, AlpSdAbs® VHH(AF647 ×4) is based on monovalent, recombinant single domain antibody to rabbit IgG kappa chain coupled to Cy3B. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG kappa, AlpSdAbs® VHH(AF647 ×4) reacts with rabbit IgG kappa chain selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.
Immunogen: Recombinant Rabbit IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: AF647(Ex=648nm, Em=671nm), 2 moles AF647 per mole VHH
Specificity: Rabbit IgG kappa chain
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles) , protect from light
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Standard immunodetection approaches use typically a primary antibody (1.Ab) which binds the protein of interest (POI) and a secondary antibody (2.Ab) that binds to the 1.Ab and carries a detection element. The complex formed by the primary antibody and the secondary antibody (1.Ab–2.Ab) is widely used because it is a cost effective and flexible approach since only the 2.Abs need to be coupled to the detection element. However, the use of this complex carries some relevant limitations. The 1.Ab–2.Ab can measure up to 30 nm, leading to a large distance between the targeted molecule and the detection element, causing the so called “linkage” or “displacement” error. While this might not influence the results in some applications (e.g. epifluorescence, ELISA or FACS), it is of major relevance for super-resolution microscopy techniques where the localization precision can be as high as 1 nm. The linkage error can be reduced by using directly labelled small affinity probes like camelid single domain antibodies (sdAbs) also known as nanobodies (Nbs), which have sizes below 3 nm.
Anti-Rabbit IgG kappa, AlpSdAbs® VHH(AF647 ×4) is designed for detecting rabbit IgG kappa chain in super-resolution microscopy techniques specifically. Anti-Rabbit IgG kappa, AlpSdAbs® VHH(AF647 ×4) is based on monovalent, recombinant single domain antibody to rabbit IgG kappa chain coupled to Cy3B. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG kappa, AlpSdAbs® VHH(AF647 ×4) reacts with rabbit IgG kappa chain selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.
Immunogen: Recombinant Rabbit IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: AF647(Ex=648nm, Em=671nm), 2 moles AF647 per mole VHH
Specificity: Rabbit IgG kappa chain
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles) , protect from light
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Standard immunodetection approaches use typically a primary antibody (1.Ab) which binds the protein of interest (POI) and a secondary antibody (2.Ab) that binds to the 1.Ab and carries a detection element. The complex formed by the primary antibody and the secondary antibody (1.Ab–2.Ab) is widely used because it is a cost effective and flexible approach since only the 2.Abs need to be coupled to the detection element. However, the use of this complex carries some relevant limitations. The 1.Ab–2.Ab can measure up to 30 nm, leading to a large distance between the targeted molecule and the detection element, causing the so called “linkage” or “displacement” error. While this might not influence the results in some applications (e.g. epifluorescence, ELISA or FACS), it is of major relevance for super-resolution microscopy techniques where the localization precision can be as high as 1 nm. The linkage error can be reduced by using directly labelled small affinity probes like camelid single domain antibodies (sdAbs) also known as nanobodies (Nbs), which have sizes below 3 nm.
Performance
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IF/Super-resolution microscopy (iStorm, etc)
The recommended dilution ratio is 1:100-1:1000.
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
The recommended dilution ratio is 1:100-1:1000.
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
