Anti-turboGFP, AlpHcAbs® Rabbit antibody

Details and Advantages

                                                Applications:
                                                ELISA,IP
                                            
                                                Reactivity:
                                                turboGFP
                                            
                                                Conjugate:
                                                Unconjugated
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-turboGFP, AlpHcAbs® Rabbit antibody is designed for detecting turboGFP fusion protein specifically. Anti-turboGFP, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-turboGFP, AlpHcAbs® Rabbit antibody is useful for detecting turboGFP fusion proteins with high sensitivity.

Immunogen: TurboGFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Unconjugated
Specificity: TurboGFP
Cross-Reactivity: No cross-reactivity with CopGFP, jellyfish GFP and derivatives
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

The dimeric green fluorescent protein TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata. It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20% sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies do not bind to TurboGFP. TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.

Performance >

ELISA: 1:5,000-1:20,000

ICC/IF: 1:200-1:1000

IP: 1-2ug/sample

Flow Cyt： 1µg for 10⁶ cells

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.