Details and Advantages
Applications:
ELISA,WB,Flow Cyt
Reactivity:
Biotin
Conjugate:
iFluor488
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Streptavidin(iFluor488) is coupled steptavidin with iFluor488. Streptavidin(iFluor488) is highly sensitive to binds biotinylated proteins in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry systems.
Conjugate: iFluor488(Ex: 495nm, Em: 519nm), 2 moles iFluor488 per mole streptavidin
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, protect from light
Background:
Streptavidin is a 53 kDa protein isolated from Streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and contains 4 subunits. Each subunit can bind one molecule of biotin. Streptavidin has strong binding properties to avidin (Kd=10-15). Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins.
Streptavidin(iFluor488) is coupled steptavidin with iFluor488. Streptavidin(iFluor488) is highly sensitive to binds biotinylated proteins in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry systems.
Conjugate: iFluor488(Ex: 495nm, Em: 519nm), 2 moles iFluor488 per mole streptavidin
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, protect from light
Background:
Streptavidin is a 53 kDa protein isolated from Streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and contains 4 subunits. Each subunit can bind one molecule of biotin. Streptavidin has strong binding properties to avidin (Kd=10-15). Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins.
Performance
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WB: 1:10,000-1:50,000
ELISA: 1:10,000-1:50,000
Flow Cyt: 1:200-1:2000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ELISA: 1:10,000-1:50,000
Flow Cyt: 1:200-1:2000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
