High lot-to-lot consistency
Increased sensitivity and higher affinity
Description:
Streptavidin-HRP is coupled steptavidin with horseradish peroxidase (HRP). Streptavidin-HRP is highly sensitive to binds biotinylated proteins in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry systems.
Background:
Streptavidin is a 53 kDa protein isolated from Streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and contains 4 subunits. Each subunit can bind one molecule of biotin. Streptavidin has strong binding properties to avidin (Kd=10-15). Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins. Streptavidin-HRP is coupled steptavidin with horseradish peroxidase (HRP), and it have a very high turnover rate, coupling high sensitivity with short reaction times.
WB: 1:10,000-1:50,000
ELISA: 1:10,000-1:50,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
