Anti-VHH, AlpHcAbs® Rabbit antibody(iFluor647)

Details and Advantages

                                                Applications:
                                                WB,ELISA,Flow Cyt
                                            
                                                Reactivity:
                                                VHH
                                            
                                                Conjugate:
                                                iFluor647
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-VHH, AlpHcAbs® Rabbit antibody(iFluor647) is designed for detecting camelid VHH antibody(Llama, Alpaca and Camel) specifically. Based on immunoelectrophoresis and/or ELISA, Anti-VHH, AlpHcAbs® Rabbit antibody(iFluor647) reacts with camelid VHH antibody selectively, no cross-reactivity with mouse, rat, rabbit, goat or human IgG.

Immunogen: Recombinant VHH antibody from Alpaca
Host: Rabbit
Isotype: Rabbit IgG
Conjugate: iFlour647(Ex: 652nm, Em: 668nm), 3 moles iFlour647 per mole IgG
Specificity: Camelid VHH antibody(Llama, Alpaca and Camel)
Cross-Reactivity: No cross-reactivity with mouse, rat, rabbit, goat or human IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles) , Protect from light.

Background:
The biological family Camelidae comprises camels (one-humped Camelus dromedarius and two-humped Camelus bactrianus), llama (Lama glama and Lama guanicoe), and vicugna (Vicugna vicugna and Vicugna pacos). Camelidae contain two kinds of IgG in serum: conventional antibodies (IgG1) containing two light chains and two heavy chains (composed of the VH, CH1, hinge, and CH2 and CH3 domains) and two types of homodimeric heavy-chain antibodies (HCAbs), IgG2 and IgG3, which comprise only H chains; each H chain contains a VHH, hinge, and CH2 and CH3 domains. The smallest intact functional antigen-binding fragment of HCAbs is the single-domain VHH, also known as a nanobody(Nb).
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

Flow Cyt: 1:100-1:1000
ELISA: 1:4000 -1:10000
WB: 1:4000 -1:10000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.