Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                Mouse IgG2b(Fcγ Fragment specific)
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP) is designed for detecting mouse IgG2b Fcγ fragment specifically. Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG2b Fcγ fragment coupled to HRP, and Anti-Mouse IgG2b(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP) reacts with the Fc fragment of mouse IgG2b selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Immunogen: Recombinant Fc region of mouse IgG2b
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Conjugate: HRP
Specificity: Mouse IgG2b (Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgG1/2a/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Affinity: ＜1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light, Stable for 12 months at -20°C

Performance >

ELISA: 1:10000-1:50000
WB: 1:10000-1:50000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.