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Anti-Llama IgG1, AlpHcAbs® Mouse antibody(Biotin)

Details and Advantages
Applications: IP,ELISA
Reactivity: Llama IgG1
Conjugate: Biotin
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

Summary >
Description:
Anti-Llama IgG1, AlpHcAbs® Mouse antibody(Biotin) is designed for detecting Llama IgG1 specifically. Anti-Llama IgG1, AlpHcAbs® Mouse antibody(Biotin) is based on monoclonal, recombinant mouse IgG1 antibody to Llama IgG1 coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Llama IgG1, AlpHcAbs® Mouse antibody(Biotin) reacts with Llama IgG1 selectively, no reactivity with Llama IgG2b or IgG2c.

Immunogen: Llama IgG1
Host: Mouse
Isotype: Mouse IgG1
Conjugate: Biotin-SP (long spacer)
Specificity: Llama IgG1(IgG1a and IgG1b)
Cross-Reactivity: No cross-reactivity with Llama IgG2b or IgG2c
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
The biological family Camelidae comprises camels (one-humped Camelus dromedarius and two-humped Camelus bactrianus), llama (Lama glama and Lama guanicoe), and vicugna (Vicugna vicugna and Vicugna pacos). Camelidae contain two kinds of IgG in serum: conventional antibodies (IgG1) containing two light chains and two heavy chains (composed of the VH, CH1, hinge, and CH2 and CH3 domains) and two types of homodimeric heavy-chain antibodies (HCAbs), IgG2 and IgG3, which comprise only H chains; each H chain contains a VHH, hinge, and CH2 and CH3 domains. The smallest intact functional antigen-binding fragment of HCAbs is the single-domain VHH, also known as a nanobody(Nb). Llama IgG contains IgG1a, IgG1b, IgG2b, IgG2c.
Performance >
ELISA:1:10000-1:50000
IP: 1-2ug/sample


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.