Anti-Human IgM, AlpHcAbs® Goat antibody(Biotin)

Details and Advantages

                                                Applications:
                                                ELISA,IP
                                            
                                                Reactivity:
                                                Human IgM
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-Human IgM, AlpHcAbs® Goat antibody(Biotin) is designed for detecting human IgM specifically. Anti-Human IgM, AlpHcAbs® Goat antibody(Biotin) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to human IgM coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgM, AlpHcAbs® Goat antibody(Biotin) reacts with human IgM selectively.

Immunogen: Human IgM
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: Biotin
Specificity: Human IgM
Cross-Reactivity: Does not bind to human IgG, IgD, IgA, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

性能 >

ELISA： 1:5000-1:20000
IP: 1-2ug/sample

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.