您的当前位置:首页 > 重组抗体

Anti-Human IgE, AlpHcAbs® Goat antibody

Details and Advantages
Applications: ICC/IF,ELISA,IP,Flow Cyt
Reactivity: Human IgE
Conjugate: Unconjugated
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description:
Anti-Human IgE, AlpHcAbs® Goat antibody is designed for detecting human IgE specifically. Anti-Human IgE, AlpHcAbs® Goat antibody is monovalent, recombinant single domain antibody fused to goat IgG Fc(mutation). Based on immunoelectrophoresis and/or ELISA, Anti-Human IgE, AlpHcAbs® Goat antibody reacts with human IgE selectively, no reactivity with human IgG, IgA, IgM, IgD.

Immunogen: Human IgE                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)    
Conjugate:  Unconjugated
Specificity: Human IgE
Cross-Reactivity: Does not bind to human IgG, IgA, IgM, IgD
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

性能 >

ELISA:       1:10000-1:50000
ICC/IF:         1:200-1:1000
IP:                1-2ug/sample
Flow Cyt:  1µg for 106 cells

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.