High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Human IgA, AlpHcAbs® Goat antibody is designed for detecting human IgA specifically. Anti-Human IgA, AlpHcAbs® Goat antibody is monovalent, recombinant single domain antibody fused to goat IgG Fc(mutation). Based on immunoelectrophoresis and/or ELISA, Anti-Human IgA, AlpHcAbs® Goat antibody reacts with human IgA selectively, no reactivity with human IgG, IgD, IgM, IgE.
Immunogen: Human IgA
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: Unconjugated
Specificity: Human IgA
Cross-Reactivity: Does not bind to human IgG, IgD, IgM, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgA is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
ELISA： 1:10000-1:50000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
