Anti-Alpaca IgG2b(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                Alpaca IgG2b(Fcγ Fragment specific)
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:
Anti-Alpaca IgG2b(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody(HRP) is designed for detecting Alpaca IgG2b specifically. Anti-Alpaca IgG2b(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to Alpaca IgG2b(Fcγ Fragment specific) coupled to HRP, and Anti-Alpaca IgG2b(Fcγ Fragment specific), AlpHcAbs® Rabbit antibody(HRP) reacts with Alpaca IgG2b selectively, no cross-reactivity with Alpaca IgG1/2c.

Immunogen: Recombinant Fc region of alpaca IgG2b
Host: Camel
Isotype: VHH domain of camel IgG3 fused to rabbit IgG Fc(mutation)
Conjugate: HRP
Specificity: Alpaca IgG2b(Fcγ Fragment specific)
Cross-Reactivity: No cross-reactivity with Alpaca IgG1/2c
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
The biological family Camelidae comprises camels (one-humped Camelus dromedarius and two-humped Camelus bactrianus), llama (Lama glama and Lama guanicoe), and vicugna (Vicugna vicugna and Vicugna pacos). Camelidae contain two kinds of IgG in serum: conventional antibodies (IgG1) containing two light chains and two heavy chains (composed of the VH, CH1, hinge, and CH2 and CH3 domains) and two types of homodimeric heavy-chain antibodies (HCAbs), IgG2 and IgG3, which comprise only H chains; each H chain contains a VHH, hinge, and CH2 and CH3 domains. The smallest intact functional antigen-binding fragment of HCAbs is the single-domain VHH, also known as a nanobody(Nb). Alpaca is also called Vicugna pacos. Alpaca IgG contains IgG1a, IgG1b, IgG2b, IgG2c and IgG3.

Performance >

ELISA：1:4000-1:10000
WB: 1:4000-1:10000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.