Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
Description:
mScarlet Nanoselector Magnetic beads have been specifically designed to bind mScarlet-fusion proteins. mScarlet Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. mScarlet Nanoselector Magnetic beads are ideal tools to isolate or purify mScarlet-fusion proteins fast and efficiently.
Ligand: Anti-mScarlet single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: mScarlet
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mScarlet.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
The fluorescent protein mScarlet is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.
For biochemical analysis including mass spectrometry and enzyme activity measurements these mScarlet-fusion proteins and their interacting factors need to be isolated fast and efficiently by immunoprecipitation using the mScarlet Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
