Details and Advantages
Applications:
IP,CHIP,MS,Purification
Reactivity:
SpyTag
Conjugate:
Magnetic beads
Advantages:
Consistent and reproducible results
Extraordinary binding, even under harsh conditions
Short Incubation (5-30 min)
概述
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Description:
SpyCatcher Magnetic beads have been specifically designed to bind SpyTag-fusion proteins. SpyCatcher Magnetic beads are based on Spycatcher covalently coupled to the surface of magnetic beads, and they are used to detect and isolate SpyTag-fusion proteins efficiently.
Ligand: SpyCatcher
Bead size: ~ 2.8 µm
Reactivity: Highly selective for biotinylated proteins.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20µg of SpyTag-fusion protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: Supplied in PBS Buffer (pH 7.2), 0.1% Tween 20.
Background:
The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag). Upon recognition, the two form a covalent isopeptide bond between the side chains of a lysine in SpyCatcher and an aspartate in SpyTag. This technology has been used, among other applications, to create covalently stabilized multi-protein complexes, for modular vaccine production, and to label proteins. The SpyTag system is versatile as the tag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins; similarly, SpyCatcher can be fused to reporter proteins such as GFP, and to epitope or purification tags.
SpyCatcher Magnetic beads have been specifically designed to bind SpyTag-fusion proteins. SpyCatcher Magnetic beads are based on Spycatcher covalently coupled to the surface of magnetic beads, and they are used to detect and isolate SpyTag-fusion proteins efficiently.
Ligand: SpyCatcher
Bead size: ~ 2.8 µm
Reactivity: Highly selective for biotinylated proteins.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20µg of SpyTag-fusion protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: Supplied in PBS Buffer (pH 7.2), 0.1% Tween 20.
Background:
The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag). Upon recognition, the two form a covalent isopeptide bond between the side chains of a lysine in SpyCatcher and an aspartate in SpyTag. This technology has been used, among other applications, to create covalently stabilized multi-protein complexes, for modular vaccine production, and to label proteins. The SpyTag system is versatile as the tag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins; similarly, SpyCatcher can be fused to reporter proteins such as GFP, and to epitope or purification tags.
性能
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Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
