Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
Description:
Biotin Nanoselector Magnetic beads have been specifically designed to bind biotinylated proteins. Biotin Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. Biotin Nanoselector Magnetic beads are ideal tools to isolate or purify biotinylated proteins fast and efficiently.
Ligand: Anti-Biotin single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 2.8µm
Reactivity: Recognizes Biotin and biotinylated antibody, protein, peptides, oligonucleotides or solid matrices.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of biotinylated protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
Biotin is widely conjugated to proteins and antibodies for biochemical assays. Avidin (streptavidin)-biotin system is commonly used for many immunoassays such as ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, and Immunohistochemistry. Anti-Biotin antibody is a better alternative to avidins to minimize background and maximize signal intensity.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
