High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Mouse IgG Fc, AlpSdAbs® VHH(VcMMAF ×4) is designed for studying on the internalization of antibodies. Anti-Mouse IgG Fc, AlpSdAbs® VHH(VcMMAF ×4) is based on recombinant single domain antibodies to mouse IgG Fc coupled to VcMMAF. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG Fc, AlpSdAbs® VHH(VcMMAF ×4) reacts with the Fc fragment of mouse IgG(including mouse IgG1, IgG2a, IgG2b) heavy chain but not with the Fab portion of mouse IgG. Anti-Mouse IgG Fc, AlpSdAbs® VHH(VcMMAF ×4) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.
Immunogen: Recombinant mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: VcMMAF(2 moles VcMMAF per mole VHH)
Specificity: Mouse IgG(Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.5mg/ml
Formation: Liquid, 10mM PBS (pH 7.4)
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Antibody Internalization Test: 2ug per 10ug test antibody (molar ratio=2:1).
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
