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Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)

Details and Advantages
Applications: ELISA,Purification,BLI,SPR
Reactivity: Mouse IgG3(Fcγ Fragment specific)
Conjugate: Biotin
Advantages:

·High lot-to-lot consistency

·Increased sensitivity and higher affinity

·Animal-free production

概述 >

Description: 

Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH (Biotin) is designed for detecting mouse IgG3 Fcγ fragment specifically. Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH (Biotin) is based on monoclonal, recombinant single domain antibody to mouse IgG3 Fc coupled to Biotin, and Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH (Biotin) reacts with the Fc fragment of mouse IgG3 selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.


Immunogen: Recombinant Fc region of mouse IgG3                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c  
Conjugate: Biotin-SP (long spacer)  
Specificity: Mouse IgG3(Fcγ fragment specific) 
Cross-Reactivity: No cross-reactivity with mouse IgG1/2a/2b, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG

Purity: Recombinant Expression and Affinity purified

Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C


性能 >

ELISA:    1:5000-1:20000
WB:       1:5000-1:20000

IP:         1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.