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Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP)

Details and Advantages
Applications: WB,ELISA
Reactivity: Human IgG(Fcγ Fragment specific)
Conjugate: HRP
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description: 

Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP) is designed for detecting human IgG Fcγ fragment specifically. Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP) is based on monoclonal, recombinant single domain antibody to human IgG Fc coupled to HRP, and Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(HRP) reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG. 


Immunogen: Recombinant Fc region of human IgG                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c  
Conjugate: HRP
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Affinity: <1nM        
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light, Stable for 12 months at -20°C

性能 >


ELISA:   1:10,000-1:50,000
WB:      1:10,000-1:50,000


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.