Details and Advantages
Applications:
WB,ELISA
Reactivity:
Human IgG(H+L)
Conjugate:
Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-Human IgG(H+L), AlpSdAbs® VHH is designed for detecting human IgG(H+L) specifically. Anti-Human IgG(H+L), AlpSdAbs® VHH is recombinant single domain antibodies derived from the variable regions of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(H+L), AlpSdAbs® VHH react with the human IgG(H+L) specifically..
Immunogen: Recombinant human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Human IgG(H+L)
Cross-Reactivity: Recognizes human IgG(H+L) specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-Human IgG(H+L), AlpSdAbs® VHH is designed for detecting human IgG(H+L) specifically. Anti-Human IgG(H+L), AlpSdAbs® VHH is recombinant single domain antibodies derived from the variable regions of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(H+L), AlpSdAbs® VHH react with the human IgG(H+L) specifically..
Immunogen: Recombinant human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Human IgG(H+L)
Cross-Reactivity: Recognizes human IgG(H+L) specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
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ELISA: 1:10,000-1:50,000
WB: 1:10,000-1:50,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
WB: 1:10,000-1:50,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
