Details and Advantages
Applications:
Isotype Control
Conjugate:
Unconjugated
Advantages:
High lot-to-lot consistency
Animal-free production
Summary
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Description:
VHH Isotype Control is designed as a negative control in VHH antibody staining applications. VHH Isotype Control has the same immunoglobulin class and subclass as the VHH antibody which is used in the experiment but lack affinity to the protein of interest. VHH Isotype Control makes experiments credible.
Isotype: VHH domain of Alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag was added at the C terminal of the VHH)
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5)
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)
Background:
Antibodies bind to a specific protein or antigen, but sometimes an antibody can bind to other proteins or cellular components in a non-specific manner. Experimental controls are critical in determining if the antibodies used in your experiment have any off-target binding capabilities. Off-target or non-specific binding by antibodies lead to false positive results, making it difficult to determine background staining from antigen-specific staining. Therefore, it is important to always include experimental controls to ensure reliable results.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
VHH Isotype Control is designed as a negative control in VHH antibody staining applications. VHH Isotype Control has the same immunoglobulin class and subclass as the VHH antibody which is used in the experiment but lack affinity to the protein of interest. VHH Isotype Control makes experiments credible.
Isotype: VHH domain of Alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag was added at the C terminal of the VHH)
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5)
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)
Background:
Antibodies bind to a specific protein or antigen, but sometimes an antibody can bind to other proteins or cellular components in a non-specific manner. Experimental controls are critical in determining if the antibodies used in your experiment have any off-target binding capabilities. Off-target or non-specific binding by antibodies lead to false positive results, making it difficult to determine background staining from antigen-specific staining. Therefore, it is important to always include experimental controls to ensure reliable results.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
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Using at a dilution as the test antibody which is used in the experiment.
