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Anti-Rabbit IgG for WB, AlpSdAbs® VHH(HRP)

Details and Advantages
Applications: WB
Reactivity: Rabbit IgG(H&L)
Conjugate: HRP
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

Summary >

Description:
Anti-Rabbit IgG for WB, AlpSdAbs® VHH(HRP) is designed for detecting rabbit IgG(H+L) in western blot experiment specifically. Anti-Rabbit IgG for WB, AlpSdAbs® VHH(HRP) is based on recombinant single domain antibodies to rabbit IgG(H+L) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG for WB, AlpSdAbs® VHH(HRP) reacts with rabbit IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant Rabbit IgG                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c    
Conjugate:  HRP
Specificity: Rabbit IgG(H+L)
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, sheep and guinea pig IgG    
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light

Background:
Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

WB:   1:10000-1:50000

WB:   1ul Nano-secondary antibody for 1ul First antibody

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.