Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP)

Details and Advantages

                                                Applications:
                                                WB
                                            
                                                Reactivity:
                                                Mouse IgG(Fcγ Fragment specific)
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                ·High lot-to-lot consistency
·Increased sensitivity and higher affinity
·Animal-free production

Summary >

Description:

Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) is designed for detecting the non-reduced mouse IgG(subtypes:IgG1, IgG2a, IgG2b, IgG3). Anti-Mouse IgG for IP, AlpSdAbs™ VHH(HRP) is based on Monovalent, recombinant single domain antibody to mouse IgG coupled to HRP, and the Anti-Mouse IgG for IP, AlpSdAbs™ VHH(HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, using Anti-mouse IgG for IP, AlpSdAbs™ VHH(HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.

Immunogen: Mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: Non-reduced mouse IgG(subtypes:IgG1, IgG2a, IgG2b, IgG3)
Cross-Reactivity: No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light

Background:

When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kDa, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Anti-mouse IgG for IP, AlpSdAbs® VHH(HRP) detects the non-reduced form of mouse IgG, no reactivity with the reduced, SDS-denatured forms. When a protein was immunoprecipitated by an antibody derived from mouse, you can still use primary antibody derived from mouse to detect this protein. Using Anti-mouse IgG for IP, AlpSdAbs® VHH(HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

WB: 1:5,000-1:10,000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.