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Anti-RFP, AlpSdAbs® VHH

Details and Advantages
Applications: WB,ELISA
Reactivity: RFP
Conjugate: Unconjugated
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description: 

Anti-RFP, AlpSdAbs® VHH is designed for detecting RFP fusion proteins specifically. Anti-RFP, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-RFP, AlpSdAbs® VHH detects the RFP selectively, no reactivity with other proteins.

Immunogen: RFP fusion protein                
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)  
Specificity: RFP
Cross-Reactivity: Recognizes mCherry, mRFP, mScarlet, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives
Affinity: Dissociation constant KD of 0.1nM      
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)


性能 >

WB:       1:5,000-1:20000
ELISA:   1:5,000-1:20000
IP:        1:100-1:500


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.