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Anti-Halo tag, AlpSdAbs® VHH

Details and Advantages
Applications: WB,ELISA
Reactivity: Halo tag
Conjugate: Unconjugated
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >

Description: 

Anti-Halo tag, AlpSdAbs® VHH is designed for detecting Halo tag fusion proteins specifically. Anti-Halo tag, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-Halo tag, AlpSdAbs® VHH detects the Halo tag selectively, no reactivity with other proteins.

Immunogen: Halo tag fused KLH                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)  
Specificity: Halo tag
Cross-Reactivity: Highly selective for Halo tag sequence
Affinity: Dissociation constant KD of 0.1nM      
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

The protein tag(Halo tag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (Halo tag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Due to the single-chain nature of sdAbs and their increased sensitivity and higher affinity to Halo tag fusion proteins, Anti-Halo Tag, AlpHcAbs® Rabbit antibody can be used to detect the Halo tag selectively, with high sensitivity and negligible non-specific background.



性能 >

WB:       1:5,000-1:20000
ELISA:   1:5,000-1:20000
IP:         1-2ug/sample


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.