Details and Advantages
Applications:
WB,ELISA
Reactivity:
mScarlet
Conjugate:
HRP
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-mScarlet, AlpHcAbs® Rabbit antibody(HRP) is designed for detecting mScarlet fusion proteins specifically. Anti-mScarlet, AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to mScarlet coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-mScarlet, AlpHcAbs® Rabbit antibody(HRP) detects the mScarlet selectively, no reactivity with other proteins.
Immunogen: mScarlet
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc(mutation)
Conjugate: HRP
Specificity: mScarlet
Cross-Reactivity: Highly selective for mScarlet
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
The fluorescent protein mScarlet is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.
Anti-mScarlet, AlpHcAbs® Rabbit antibody(HRP) is designed for detecting mScarlet fusion proteins specifically. Anti-mScarlet, AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to mScarlet coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-mScarlet, AlpHcAbs® Rabbit antibody(HRP) detects the mScarlet selectively, no reactivity with other proteins.
Immunogen: mScarlet
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc(mutation)
Conjugate: HRP
Specificity: mScarlet
Cross-Reactivity: Highly selective for mScarlet
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
The fluorescent protein mScarlet is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.
Performance
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WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ELISA: 1:5,000-1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
