Details and Advantages
Applications:
WB,ELISA,IP,ICC/IF,Flow Cyt
Reactivity:
V5 tag
Conjugate:
Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
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Description:
Anti-V5 tag, AlpHcAbs® Human antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Human antibody is monovalent, recombinant single domain antibody fused to Human IgG1 Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Human antibody reacts with the V5 tag sequence(GKPIPNPLLGLDST) selectively, no reactivity with other proteins.
Immunogen: V5 tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: V5 tag sequence(GKPIPNPLLGLDST)
Cross-Reactivity: Highly selective for V5 tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)
Background:
The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Anti-V5 tag, AlpHcAbs® Human antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Human antibody is monovalent, recombinant single domain antibody fused to Human IgG1 Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Human antibody reacts with the V5 tag sequence(GKPIPNPLLGLDST) selectively, no reactivity with other proteins.
Immunogen: V5 tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: V5 tag sequence(GKPIPNPLLGLDST)
Cross-Reactivity: Highly selective for V5 tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)
Background:
The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
性能
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ELISA: 1:5000 -1:20000
WB: 1:5000 -1:20000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt: 1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
WB: 1:5000 -1:20000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt: 1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
