Details and Advantages
Applications:
ELISA,WB,ICC/IF,Flow Cyt
Reactivity:
Rat IgG(H+L)
Conjugate:
iFluor647
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
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Description:
Anti-Rat IgG(H+L), AlpHcAbs® Goat antibody(iFluor647) is designed for detecting rat IgG specifically. Anti-Rat IgG(H+L), AlpHcAbs® Goat antibody(iFluor647) is based on recombinant, goat IgG Fc fused single domain antibody to rat IgG coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Rat IgG(H+L), AlpHcAbs® Goat antibody(iFluor647) reacts with rat IgG selectively.
Immunogen: Rat IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: iFluor647(Ex: 652nm, Em: 668nm), 3 moles iFluor647 per mole IgG
Specificity: Rat IgG(H+L)
Cross-Reactivity: Does not bind to mouse IgG, rabbit IgG, goat IgG, human IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Background:
There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from rat. Each isotype has a different heavy chain. Rat IgG consists of four subclasses-IgG1, IgG2a, IgG2b, IgG2c. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Anti-Rat IgG(H+L), AlpHcAbs® Goat antibody(iFluor647) is designed for detecting rat IgG specifically. Anti-Rat IgG(H+L), AlpHcAbs® Goat antibody(iFluor647) is based on recombinant, goat IgG Fc fused single domain antibody to rat IgG coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Rat IgG(H+L), AlpHcAbs® Goat antibody(iFluor647) reacts with rat IgG selectively.
Immunogen: Rat IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: iFluor647(Ex: 652nm, Em: 668nm), 3 moles iFluor647 per mole IgG
Specificity: Rat IgG(H+L)
Cross-Reactivity: Does not bind to mouse IgG, rabbit IgG, goat IgG, human IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Background:
There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from rat. Each isotype has a different heavy chain. Rat IgG consists of four subclasses-IgG1, IgG2a, IgG2b, IgG2c. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
性能
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Flow Cyt: 1:200-1:2000
ICC/IF： 1:200-1:2000
ELISA: 1:5000 -1:20000
WB: 1:5000 -1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ICC/IF： 1:200-1:2000
ELISA: 1:5000 -1:20000
WB: 1:5000 -1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
