Details and Advantages
Applications:
ELISA,WB,ICC/IF,Flow Cyt
Reactivity:
Mouse IgG2a(Fcγ Fragment specific)
Conjugate:
iFluor488
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
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Description:
Anti-Mouse IgG2a(Fcγ Fragment specific), AlpHcAbs® Goat antibody(iFluor488) is designed for detecting mouse IgG2a Fcγ fragment specifically. Anti-Mouse IgG2a(Fcγ Fragment specific), AlpHcAbs® Goat antibody(iFluor488) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG2a Fcγ fragment coupled to iFluor488. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG2a(Fcγ Fragment specific), AlpHcAbs® Goat antibody(iFluor488) reacts with the Fc fragment of mouse IgG2a selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Immunogen: Recombinant Fc region of mouse IgG2a
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Conjugate: iFluor488(Ex: 495nm, Em: 519nm), 3 moles iFluor488 per mole IgG
Specificity: Mouse IgG2a (Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgG1/2b/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-Mouse IgG2a(Fcγ Fragment specific), AlpHcAbs® Goat antibody(iFluor488) is designed for detecting mouse IgG2a Fcγ fragment specifically. Anti-Mouse IgG2a(Fcγ Fragment specific), AlpHcAbs® Goat antibody(iFluor488) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG2a Fcγ fragment coupled to iFluor488. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG2a(Fcγ Fragment specific), AlpHcAbs® Goat antibody(iFluor488) reacts with the Fc fragment of mouse IgG2a selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Immunogen: Recombinant Fc region of mouse IgG2a
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Conjugate: iFluor488(Ex: 495nm, Em: 519nm), 3 moles iFluor488 per mole IgG
Specificity: Mouse IgG2a (Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgG1/2b/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
性能
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Flow Cyt: 1:200-1:2000
ICC/IF： 1:200-1:2000
ELISA: 1:10000 -1:50000
WB: 1:10000 -1:50000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ICC/IF： 1:200-1:2000
ELISA: 1:10000 -1:50000
WB: 1:10000 -1:50000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
