Details and Advantages
Applications:
ELISA,WB
Reactivity:
Human IgG(Fcγ Fragment specific)
Conjugate:
HRP
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
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Description:
Anti-Human IgG-Fc PK, AlpHcAbs® Goat antibody(HRP) is designed for detecting human IgG specifically. Anti-Human IgG-Fc PK, AlpHcAbs® Goat antibody(HRP) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to human IgG coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG-Fc PK, AlpHcAbs® Goat antibody(HRP) binds to the Fc part of all four human IgG subclasses without cross-binding to rabbit, mouse, rat, goat, rhesus, and cynomolgus monkey IgG. Anti-Human IgG-Fc PK, AlpHcAbs® Goat antibody(HRP) is a useful tool to detect, quantitate, and characterize all human IgG antibodies(subclasses 1 to 4), recombinant human IgG antibodies, human IgG-derived Fc-fusion proteins in, for instance, non-human plasma and/or serum samples like mouse, rat , rhesus, and cynomolgus monkey, thereby making it extremely suitable for setting up pharmacokinetics (PK) assays.
Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: HRP
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: No Cross-reactivity to rabbit, mouse, rat, goat, rhesus, and cynomolgus monkey IgG.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Anti-Human IgG-Fc PK, AlpHcAbs® Goat antibody(HRP) is designed for detecting human IgG specifically. Anti-Human IgG-Fc PK, AlpHcAbs® Goat antibody(HRP) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to human IgG coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG-Fc PK, AlpHcAbs® Goat antibody(HRP) binds to the Fc part of all four human IgG subclasses without cross-binding to rabbit, mouse, rat, goat, rhesus, and cynomolgus monkey IgG. Anti-Human IgG-Fc PK, AlpHcAbs® Goat antibody(HRP) is a useful tool to detect, quantitate, and characterize all human IgG antibodies(subclasses 1 to 4), recombinant human IgG antibodies, human IgG-derived Fc-fusion proteins in, for instance, non-human plasma and/or serum samples like mouse, rat , rhesus, and cynomolgus monkey, thereby making it extremely suitable for setting up pharmacokinetics (PK) assays.
Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: HRP
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: No Cross-reactivity to rabbit, mouse, rat, goat, rhesus, and cynomolgus monkey IgG.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
性能
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ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
WB: 1:5000-1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
