Human His tag Antibody Plate

Description:
Human His Tag Antibody Plate is a 96-well microtiter plate coated with Anti-His tag, AlpHcAbs® Human antibody. The product is developed for rapid capture of His-tagged protein in different samples, including His-tagged proteins from Ecoli, yeast and mammalian extracts and cell culture supernatant. The plate can be applied to many assays, from direct His-tagged protein detection and screening, to more comprehensive protein-protein interaction assays. Human His Tag Antibody Plate can bind target proteins with high specificity and capacity. Its capacity and sensitivity varies, depending on protein size, structure and solution environment. Generally, the plate is more sensitive to capture His-tagged protein with lower molecular weight. Any detection reagent, which can recognize with human IgG, should not be applied for the plate, such as anti-human IgG antibodies and protein A/G.


Pre-coated Antibody: Anti-His tag, AlpHcAbs® Human antibody
Specificity: N-terminal/C-terminal/internal His-tagged protein
Sensitivity: 1 ng/well
Capacity: 100~300 ng/well
Reagents Compatibility: Compatible with common used reagents
Storage：Human His Tag Antibody Plate should be shipped on ice pack. The unopened plate is stable for at least 2 years when stored at 2-8 °C. The product is more stable when stored at -20 °C. The opened plate should be used within one week.

Protein Quantification
Protein Expression Screening
Immuno-capture of DYKDDDDK-tagged protein complex

All the reagents should be equilibrated to room temperature (20-25 °C) before test.
This manual gives general protocols for different assays. The user should optimize the protocol to achieve ideal test result.