Streptavidin-Magnetic beads

Details and Advantages

                                                Applications:
                                                IP,CHIP,MS,Purification
                                            

                                                Reactivity:
                                                Biotin
                                            

                                                Conjugate:
                                                Magnetic beads
                                            

                                                Advantages:
                                                · Consistent and reproducible results
· No heavy & light antibody chains 
· Extraordinary binding, even under harsh conditions
· High affinity 
· Short Incubation (5-30 min)

                                            

Summary >

Description:

Streptavidin Magnetic beads have been specifically designed to bind biotinylated proteins. Streptavidin Magnetic beads are based on Streptavidin covalently coupled to the surface of Magnetic beads, and they are used to detect and isolate biotinylated proteins efficiently.

Ligand:Streptavidin
Bead size: ~2.8µm
Reactivity: Highly selective for biotinylated proteins.
Binding capacity: High binding capacity for biotinylated proteins.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: Supplied in PBS Buffer (pH 7.2), 0.1% Tween 20.

Background:

Streptavidin is a 53 kDa protein isolated from Streptomyces avidinii. Streptavidin contains 4 subunits. Each subunit can bind one molecule of biotin. Streptavidin has strong binding properties to avidin(Kd=10-15). Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins. Streptavidin Magnetic beads are coupled streptavidin with magnetic beads, and they feature high affinity and superior capacity for biotinylated proteins.

Performance >

Immunoprecipitation (IP)/Co-IP
Mass spectrometry (MS)
Enzyme activity measurements