Details and Advantages
Applications:
IP,CHIP,MS,Purification
Reactivity:
His tag
Conjugate:
Magnetic beads
Advantages:
Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
概述
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Description:
C-His tag Nanoselector Magnetic beads have been specifically designed to bind His tag-fusion proteins. C-His tag Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. C-His tag Nanoselector Magnetic beads are ideal tools to isolate or purify His tag-fusion proteins fast and efficiently.
Ligand: Anti-His tag(C-terminal) single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 2.8µm
Reactivity: The C-His tag Nanoselector recognizes the His-tag sequence motif HHHHHH at the C-terminus of the fusion protein.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20µg of recombinant GFP-His protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
His-tag, or polyhistidine tag, comprises a consecutive series of six to ten histidine residues. The size of the His tag is small and it hardly affects the functionality of the fusion protein. His tag is widely used for many recombinant proteins to facilitate purification, and allows researchers to purify the protein of interest from cell lysate or mixture of many proteins easily .
His tag Nanoselector Magnetic beads has been specifically designed to bind His tag. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. His tag Nanoselector Magnetic beads thus features high affinity and superior capacity for His tag fusion proteins while showing negligible non-specific background. His tag Nanoselector Magnetic beads is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
C-His tag Nanoselector Magnetic beads have been specifically designed to bind His tag-fusion proteins. C-His tag Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. C-His tag Nanoselector Magnetic beads are ideal tools to isolate or purify His tag-fusion proteins fast and efficiently.
Ligand: Anti-His tag(C-terminal) single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 2.8µm
Reactivity: The C-His tag Nanoselector recognizes the His-tag sequence motif HHHHHH at the C-terminus of the fusion protein.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20µg of recombinant GFP-His protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
His-tag, or polyhistidine tag, comprises a consecutive series of six to ten histidine residues. The size of the His tag is small and it hardly affects the functionality of the fusion protein. His tag is widely used for many recombinant proteins to facilitate purification, and allows researchers to purify the protein of interest from cell lysate or mixture of many proteins easily .
His tag Nanoselector Magnetic beads has been specifically designed to bind His tag. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. His tag Nanoselector Magnetic beads thus features high affinity and superior capacity for His tag fusion proteins while showing negligible non-specific background. His tag Nanoselector Magnetic beads is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
性能
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Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
