Anti-Mouse IgG kappa chain, AlpSdAbs® VHH(VcMMAF ×4)

Details and Advantages
Applications: Internalization Test
Reactivity: Mouse IgG kappa
Conjugate: VcMMAF
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

Summary >
Description:
Anti-Mouse IgG kappa chain, AlpSdAbs® VHH(VcMMAF ×4) is designed for studying on the internalization of antibodies. Anti-Mouse IgG kappa chain, AlpSdAbs® VHH(VcMMAF ×4) is based on recombinant single domain antibodies to mouse IgG kappa chain coupled to VcMMAF. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG kappa chain, AlpSdAbs® VHH(VcMMAF ×4) reacts with the mouse IgG kappa chain. Anti-Mouse IgG kappa chain, AlpSdAbs® VHH(VcMMAF ×4) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.

Immunogen: Recombinant mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: VcMMAF(2 moles VcMMAF per mole VHH)
Specificity: Mouse IgG kappa chain
Cross-Reactivity: No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.4)
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance >
Antibody Internalization Test: 2ug per 10ug test antibody (molar ratio=2:1).


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.