Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(iFluor594)

Details and Advantages

                                                Applications:
                                                WB,ICC/IF,ELISA,Flow Cyt
                                            
                                                Reactivity:
                                                Human IgG(Fcγ Fragment specific)
                                            
                                                Conjugate:
                                                iFluor594
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(iFluor594) is designed for detecting human IgG Fcγ fragment specifically. Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant single domain antibody to human IgG Fc coupled to iFluor594. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(iFluor594) reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG.

Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor594(Ex: 592nm, Em: 614nm)
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles) , Protect from light.

Background:
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

性能 >

Flow Cyt: 1:200-1:2000
ICC/IF： 1:200-1:2000
ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
Super-resolution microscopy

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.