Details and Advantages
Applications:
ELISA,IP
Reactivity:
Human IgG(Fcγ Fragment specific)
Conjugate:
Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
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Description:
Anti-Human IgG-Fc PK, AlpSdAbs® VHH is designed for detecting human IgG specifically. Anti-Human IgG-Fc PK, AlpSdAbs® VHH is monovalent, recombinant single domain antibody derived from the variable regions of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG-Fc PK, AlpSdAbs® VHH binds to the Fc part of all four human IgG subclasses without cross-binding to rabbit, mouse, rat, goat, rhesus, and cynomolgus monkey IgG. Anti-Human IgG-Fc PK, AlpSdAbs® VHH is a useful tool to detect, quantitate, and characterize all human IgG antibodies(subclasses 1 to 4), recombinant human IgG antibodies, human IgG-derived Fc-fusion proteins in, for instance, non-human plasma and/or serum samples like mouse, rat , rhesus, and cynomolgus monkey, thereby making it extremely suitable for setting up pharmacokinetics (PK) assays.
Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: No Cross-reactivity to rabbit, mouse, rat, goat, rhesus, and cynomolgus monkey IgG.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-Human IgG-Fc PK, AlpSdAbs® VHH is designed for detecting human IgG specifically. Anti-Human IgG-Fc PK, AlpSdAbs® VHH is monovalent, recombinant single domain antibody derived from the variable regions of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG-Fc PK, AlpSdAbs® VHH binds to the Fc part of all four human IgG subclasses without cross-binding to rabbit, mouse, rat, goat, rhesus, and cynomolgus monkey IgG. Anti-Human IgG-Fc PK, AlpSdAbs® VHH is a useful tool to detect, quantitate, and characterize all human IgG antibodies(subclasses 1 to 4), recombinant human IgG antibodies, human IgG-derived Fc-fusion proteins in, for instance, non-human plasma and/or serum samples like mouse, rat , rhesus, and cynomolgus monkey, thereby making it extremely suitable for setting up pharmacokinetics (PK) assays.
Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: No Cross-reactivity to rabbit, mouse, rat, goat, rhesus, and cynomolgus monkey IgG.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
性能
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ELISA： 1:5000-1:20000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
