Anti-Mouse IgG for WB, AlpSdAbs® VHH(HRP)

Details and Advantages

                                                Applications:
                                                WB
                                            
                                                Reactivity:
                                                Mouse IgG(H&L)
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-Mouse IgG for WB, AlpSdAbs® VHH(HRP) is designed for detecting mouse IgG(H+L) in western blot experiment specifically. Anti-Mouse IgG for WB, AlpSdAbs® VHH(HRP) is based on recombinant single domain antibodies to mouse IgG(H+L) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG for WB, AlpSdAbs® VHH(HRP) detects the mouse IgG1, mouse IgG2a, mouse IgG2b and mouse IgG3, no reactivity with rabbit, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: Mouse IgG(H+L)
Cross-Reactivity: No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/mL
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.

Background:
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

性能 >

WB: 1:10000-1:50000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.